LIVE BORN LIZARDS SOURCE CALCIUM THROUGH CHORIOALLANTOIC MEMBRANE
نویسندگان
چکیده
منابع مشابه
Calcium-binding protein of the chick chorioallantoic membrane. II. Vitamin K-dependent expression
A simple method was devised for the maintenance of the chorioallantoic membrane (CAM) of chick embryos in organ culture. Explants of CAM survived for up to 5 days in this system and retained the characteristic three-layered morphology (ectoderm, mesoderm, and endoderm). Induction of the CAM calcium-binding protein (CaBP) by effectors of calcium metabolism was studied in these organ cultures. Vi...
متن کاملTransepithelial calcium transport in the chick chorioallantoic membrane. I. Isolation and characterization of chorionic ectoderm cells.
The chicken eggshell supplies approximately 80% of the calcium found in the hatchling chick. The mobilization of eggshell calcium into the developing embryo involves the transepithelial transport of large amounts of calcium in a development-specific manner. The cells responsible for the transport of eggshell calcium into the embryonic circulation are the ectodermal cells of the chorioallantoic ...
متن کاملCalcium-transport function of the chick embryonic chorioallantoic membrane. I. In vivo and in vitro characterization.
During chick embryonic development, the chorioallantoic membrane (CAM) is responsible for the mobilization of shell calcium into the embryonic circulation. The calcium-transport function of the CAM was studied here by measuring CAM calcium uptake in vivo and in vitro. The in vivo technique involved the use of an uptake chamber constructed on top of the CAM in situ. The in vitro methods included...
متن کاملMobilization of shell calcium by the chick chorioallantoic membrane in vitro.
Two explants of shell were removed from each of several fertile eggs of domestic fowl at different times during incubation. The chorioallantoic membrane (CAM) was removed from one of the explants (SHELL ONLY) and was left in situ on the other (SHELL+CAM). Explants were cultured for 24, 48 or 96 h at 37 degrees C and 5% CO2 in air in individual Petri dishes containing Dulbecco's modified Eagle's...
متن کاملCalcium-binding protein of the chick chorioallantoic membrane. I. Immunohistochemical localization
The preparation of a specific antiserum (anti-CaBP) against the calcium-binding protein (CaBP) of the chorioallantoic membrane (CAM) is described. The anti-CaBP appeared to be specific for the CaBP by immunodiffusion and immunoelectrophoresis. Application of the anti-CaBP in immunofluorescence histochemistry revealed that the CaBP is present in the CAM only at developmental ages corresponding w...
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ژورنال
عنوان ژورنال: Journal of Experimental Biology
سال: 2011
ISSN: 1477-9145,0022-0949
DOI: 10.1242/jeb.063784